沪鼎产品文献:植物MgCh,Rubisco,ELISA试剂盒引用文献

【文献标题】Transcriptome profiling of genes involved in photosynthesis in Elaeagnus angustifolia L. under salt stress 
【作者】J. LIN, J.P. LI, F. YUAN,et.al
【作者单位】山东师范大学（Shandong Normal University）
【文献中引用产品】
植物镁螯合酶(MgCh) ELISA试剂盒
植物Rubisco活化酶(RCA)ELISA试剂盒
【关键词】biomass; greening; ion concentrations; photosynthetic parameters; plant height. 
【DOI】doi.org/10.1007/s11099-018-0824-6
【影响因子(IF)】2.3
【出版期刊】《PHOTOSYNTHETICA》
【产品原文引用】
Protein content and activity of magnesium chelatase and Rubisco:
Magnesium chelatase and Rubisco were prepared by first freezing a 0.2-g leaf sample in liquid nitrogen to prevent proteolytic activity, followed by grinding with 2 mL of extraction buffer (100 mM phosphate buffer, pH 7.2, containing 0.1 mM EDTA, and 2 mM ascorbic acid). The homogenate was centrifuged for 15 min at 10,000 rpm, and the supernatant was used to measure the protein content (ng L–1), magnesium chelatase activity [pmol(Mg-Deutero) min–1 mg–1(protein)] and Rubisco activity [μmol min–1 mg–1(protein)]. Magnesium chelatase and Rubisco were measured using a  magnesium  chelatase content kit and a Rubisco content kit (double antibody sandwich method, Huding Biological Technology Co., Shanghai, China) (3 replicates per treatment). 

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